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fluorescein labeled lens culinaris agglutinin (lca)  (Vector Laboratories)


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    Vector Laboratories fluorescein labeled lens culinaris agglutinin (lca)
    Fluorescein Labeled Lens Culinaris Agglutinin (Lca), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescein+labeled+lca/Fluorescein+labeled+Lens+Culinaris+Agglutinin+(LCA)/custom%40fl-1041%4042710613
    Average 93 stars, based on 78 article reviews
    fluorescein labeled lens culinaris agglutinin (lca) - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: CP40 from Corynebacterium pseudotuberculosis is an endo-β- N -acetylglucosaminidase
    Article Snippet: Lectin blot was carried out using a Trans-Blot Turbo Transfer system (Bio-Rad Laboratories). .. The membranes were blocked in lectin buffer (10 mM Hepes, 0.15 M NaCl, 0.1 % Tween 20, 0.01 mM MnCl 2 and 0.1 mM CaCl 2 , pH 7.5) for 20 min and incubated with 2 μg ml −1 of fluorescein labeled LCA ( Lens culinaris agglutinin) (Vector Laboratories, Burlingame, CA) in lectin buffer for 45 min. .. The membranes were washed three times with lectin buffer and visualized using a Chemidoc XRS (Bio-Rad Laboratories).

    Labeling:

    Article Title: CP40 from Corynebacterium pseudotuberculosis is an endo-β- N -acetylglucosaminidase
    Article Snippet: Lectin blot was carried out using a Trans-Blot Turbo Transfer system (Bio-Rad Laboratories). .. The membranes were blocked in lectin buffer (10 mM Hepes, 0.15 M NaCl, 0.1 % Tween 20, 0.01 mM MnCl 2 and 0.1 mM CaCl 2 , pH 7.5) for 20 min and incubated with 2 μg ml −1 of fluorescein labeled LCA ( Lens culinaris agglutinin) (Vector Laboratories, Burlingame, CA) in lectin buffer for 45 min. .. The membranes were washed three times with lectin buffer and visualized using a Chemidoc XRS (Bio-Rad Laboratories).

    Article Title: TSTA3 facilitates esophageal squamous cell carcinoma progression through regulating fucosylation of LAMP2 and ERBB2
    Article Snippet: Subsequently, the cells were analyzed by flow cytometry (FACS-Calibur; BD Biosciences, Franklin Lakes, NJ, USA). .. After cell adhesion, 10 μg FITC-UEA (Sigma) or Fluorescein labeled LCA (Vector lab) and appropriate amount of PBS were added to each well until the total volume was 1000 μl. .. After cell adhesion, 10 μg FITC-UEA (Sigma) or Fluorescein labeled LCA (Vector lab) and appropriate amount of PBS were added to each well until the total volume was 1000 μl.

    other:

    Article Title: Quantitative glycoproteomics analysis identifies novel FUT8 targets and signaling networks critical for breast cancer cell invasiveness.
    Article Snippet: For lectin blot analysis, membranes were blocked with PBS (pH 7.5) containing 0.1% gelatin and 0.05% Tween 20, detected with biotinylated Lens culinaris agglutinin (LCA) lectin (Vector Laboratories, Burlingame, CA), then incubated with horseradish peroxidase-conjugated streptavidin (Vector Laboratories).



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    (A) Uniform manifold approximation and projection displaying adipocyte identities and expression densities for main marker genes in the STx data. (B) Dot plots displaying the scaled average expression (expr.) profiles of the top three marker genes for each adipocyte identity in the present work and a previous study. (C) Abundance (abun.) and depot enrichments (scaled proportions, prop.) for the three main adipocyte identities. Significant enrichments ( p < 0.05 using likelihood ratio test, LRT) are indicated. (D) Heatmaps displaying expressional enrichments of Adipo SAA markers across depots. Results are shown as scaled average expression for bulk transcriptomics and proteomics from Krieg et al., as well as STx and snRNA-seq in the main (1) and validation (2) cohorts. (E) Quantification of CES1 and <t>lectin</t> (LCA) normalized mean fluorescence intensity based on tissue sections from six individuals imaged using confocal microscopy. Asterisks denote statistically significant differences (* p < 0.05) comparing depots using one-way repeated measures ANOVA and Tukey’s multiple comparisons test. Error bars are standard error of the mean. (F) Representative image of epiploic WAT stained with LCA and an antibody directed against CES1. Scale bars, 200 μm (merge) and 100 μm (inlay). epi, epiploic; mc, mesocolic; ms, mesenteric; om, omental; sc, subcutaneous.
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    (A) Uniform manifold approximation and projection displaying adipocyte identities and expression densities for main marker genes in the STx data. (B) Dot plots displaying the scaled average expression (expr.) profiles of the top three marker genes for each adipocyte identity in the present work and a previous study. (C) Abundance (abun.) and depot enrichments (scaled proportions, prop.) for the three main adipocyte identities. Significant enrichments ( p < 0.05 using likelihood ratio test, LRT) are indicated. (D) Heatmaps displaying expressional enrichments of Adipo SAA markers across depots. Results are shown as scaled average expression for bulk transcriptomics and proteomics from Krieg et al., as well as STx and snRNA-seq in the main (1) and validation (2) cohorts. (E) Quantification of CES1 and <t>lectin</t> (LCA) normalized mean fluorescence intensity based on tissue sections from six individuals imaged using confocal microscopy. Asterisks denote statistically significant differences (* p < 0.05) comparing depots using one-way repeated measures ANOVA and Tukey’s multiple comparisons test. Error bars are standard error of the mean. (F) Representative image of epiploic WAT stained with LCA and an antibody directed against CES1. Scale bars, 200 μm (merge) and 100 μm (inlay). epi, epiploic; mc, mesocolic; ms, mesenteric; om, omental; sc, subcutaneous.
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    (A) Uniform manifold approximation and projection displaying adipocyte identities and expression densities for main marker genes in the STx data. (B) Dot plots displaying the scaled average expression (expr.) profiles of the top three marker genes for each adipocyte identity in the present work and a previous study. (C) Abundance (abun.) and depot enrichments (scaled proportions, prop.) for the three main adipocyte identities. Significant enrichments ( p < 0.05 using likelihood ratio test, LRT) are indicated. (D) Heatmaps displaying expressional enrichments of Adipo SAA markers across depots. Results are shown as scaled average expression for bulk transcriptomics and proteomics from Krieg et al., as well as STx and snRNA-seq in the main (1) and validation (2) cohorts. (E) Quantification of CES1 and <t>lectin</t> (LCA) normalized mean fluorescence intensity based on tissue sections from six individuals imaged using confocal microscopy. Asterisks denote statistically significant differences (* p < 0.05) comparing depots using one-way repeated measures ANOVA and Tukey’s multiple comparisons test. Error bars are standard error of the mean. (F) Representative image of epiploic WAT stained with LCA and an antibody directed against CES1. Scale bars, 200 μm (merge) and 100 μm (inlay). epi, epiploic; mc, mesocolic; ms, mesenteric; om, omental; sc, subcutaneous.
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    Image Search Results


    (A) Uniform manifold approximation and projection displaying adipocyte identities and expression densities for main marker genes in the STx data. (B) Dot plots displaying the scaled average expression (expr.) profiles of the top three marker genes for each adipocyte identity in the present work and a previous study. (C) Abundance (abun.) and depot enrichments (scaled proportions, prop.) for the three main adipocyte identities. Significant enrichments ( p < 0.05 using likelihood ratio test, LRT) are indicated. (D) Heatmaps displaying expressional enrichments of Adipo SAA markers across depots. Results are shown as scaled average expression for bulk transcriptomics and proteomics from Krieg et al., as well as STx and snRNA-seq in the main (1) and validation (2) cohorts. (E) Quantification of CES1 and lectin (LCA) normalized mean fluorescence intensity based on tissue sections from six individuals imaged using confocal microscopy. Asterisks denote statistically significant differences (* p < 0.05) comparing depots using one-way repeated measures ANOVA and Tukey’s multiple comparisons test. Error bars are standard error of the mean. (F) Representative image of epiploic WAT stained with LCA and an antibody directed against CES1. Scale bars, 200 μm (merge) and 100 μm (inlay). epi, epiploic; mc, mesocolic; ms, mesenteric; om, omental; sc, subcutaneous.

    Journal: Cell metabolism

    Article Title: Cytoarchitectural multi-depot profiling reveals immune-metabolic crosstalk in human colon-associated adipose tissue

    doi: 10.1016/j.cmet.2025.12.008

    Figure Lengend Snippet: (A) Uniform manifold approximation and projection displaying adipocyte identities and expression densities for main marker genes in the STx data. (B) Dot plots displaying the scaled average expression (expr.) profiles of the top three marker genes for each adipocyte identity in the present work and a previous study. (C) Abundance (abun.) and depot enrichments (scaled proportions, prop.) for the three main adipocyte identities. Significant enrichments ( p < 0.05 using likelihood ratio test, LRT) are indicated. (D) Heatmaps displaying expressional enrichments of Adipo SAA markers across depots. Results are shown as scaled average expression for bulk transcriptomics and proteomics from Krieg et al., as well as STx and snRNA-seq in the main (1) and validation (2) cohorts. (E) Quantification of CES1 and lectin (LCA) normalized mean fluorescence intensity based on tissue sections from six individuals imaged using confocal microscopy. Asterisks denote statistically significant differences (* p < 0.05) comparing depots using one-way repeated measures ANOVA and Tukey’s multiple comparisons test. Error bars are standard error of the mean. (F) Representative image of epiploic WAT stained with LCA and an antibody directed against CES1. Scale bars, 200 μm (merge) and 100 μm (inlay). epi, epiploic; mc, mesocolic; ms, mesenteric; om, omental; sc, subcutaneous.

    Article Snippet: Fluorescein-labeled lectin (1:200, FL-1041, Vector labs) or Dylight649-labeled lectin (1:200, DL-1048–1, Vector labs), together with Hoechst33342 (1:2000, ab228551, Abcam), was applied on the slides for 20 minutes.

    Techniques: Expressing, Marker, Transcriptomics, Biomarker Discovery, Fluorescence, Confocal Microscopy, Staining